This step might require concentrating the yeast by centrifugation and removal of a part of the supernatant.Įstimated bench time: 5 minutes. Mix in 33 ml of yeast solution at OD 600 of 12. Since sterility must be guaranteed when testing, first make the sodium alginate solution with 2 g in 67 ml of the media in which your cells grow. If not, slow down the rotation until the magnet sinks and slowly resume 700 rpm rotation.Īlternative steps for testing (additional 20-40 minutes) Make sure the magnet stays at the bottom of the liquid solution. Mix alginic acid sodium salt powder into 100 ml cell dense media. MaterialsĢ g alginic acid sodium salt powder (sodium alginate)
Purpose: This 2% solution is the basis for the yeast beads. Sodium-alginate cell solution preparationĮstimated bench time: 5 minutes. The protocol below is solely for making beads that contain cells which are to be used capturing devices: it does not respect sterility rules.
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